Deep Vent Pol (ssDNA)

Fiberhead stern100 at mail.concentric.net
Thu Feb 6 17:16:29 EST 1997


On Thu, Feb 6, 1997 10:47 AM, Moez TORKI <mailto:torki at BIO.GRENET.FR>
wrote:
>      Dear reserchers,
>
>        I've got some troubles with Deep Vent Pol.
>

Can't say but Deep Vent has agressive exonuc activity that can degrade
primers.  Try the exo- version first.  If you can get that to work, do
a units titration of exo+ and keep the extension time to a minimum.
We use this enzyme routinely and like it, but it can be tricky.

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