Cloning problem

[Brendan Scott]

>Hi,
>Have you tried fractionating by directly excising the appropriate area of
>smearing from an agarose gel and gel extracting the fragments?

>cheers
>Nick

Yes, we do this as a matter of course after the partial digest, in both the
"dirty" approach (the one that works fine) and the "cleaner" approach (the
one that doesn't).

Cheers,
Brendan

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Brendan Scott BSc. PGDip(Hons. (Parasitology)) Qld

Institute of Dental Research            Department of Pathology
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