Silver Staining Reveals Contaminating Bands in Sample Buffer

Huang Ke-xue khuang at CHEMVX.CHEM.TAMU.EDU
Fri Feb 7 11:35:27 EST 1997


>Tom Buckley wrote:
>> 
>> Dear Readers,
>>         I am looking for a way to get rid of 2 contaminating bands in one
>> of the reagents in SDS Page sample buffer. These bands are visible on
>> silver stained gels. I would appreciate hearing from anyone who has had a
>> similar experience and has solved this problem. Email replies can be sent
>> to klnelson at uvic.ca
>> Thanks
>
>
>Are those bands in the 60 - 66kDa range?  If yes, then you have just
>re-discovered a famous beta-mercaptoethanol effect, so far,
>unexplainable in its chemistry. 
>
>Your sample buffer or your BME or both are probably to old. Therefore I
>would recommend to prepare fresh buffer. When BME oxidizes it gets
>yellow and has to be discarded.
>
>Achim

I think Dr. Paul wrote a summary about this problem in TIBS (1996). You
better check the references and do some real work by yourself.

Huang




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