electrophoresis at pH 8,0

Alexander N. Kukushkin kan at mmcd.cyt.ras.spb.ru
Fri Feb 7 11:34:49 EST 1997


>   Does anybody know about a protein-electrophoresis-system that is able to
>   resolve and focus satisfying at pH 8.0. I tried Tris-Acetate-System
>   (0.05 M) and the results were not satisfying (bands do not focus well
>   enough). Is there a system that makes a stacking like the
>   Laemmli-System?
>
Why not use Tris-Glycine buffer with pH 8.0.
Alex



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