Q: purifying lambda phage from extraneous DNA

Hiranya Roychowdhury hroychow at NMSU.EDU
Fri Feb 7 10:58:26 EST 1997


At 05:41 AM 2/7/97 GMT, Greg Stuart wrote:
>I am looking for suggestions on how to purify (or separate) lambda
>phage particles from DNA left-over from an in vitro packaging
>reaction. Any ideas? Thanks, Greg  :)
>Greg Stuart    gstuart at uvic.ca
>Centre for Environmental Health .   .*#*.            .*#*.   .*#*.
>Department of Biology    * | | | * | | | *        * | | | * | | | *
>University of Victoria * | | | *   * | | | *    * | | | *   * | | | *
>P.O. Box 3020         *  | | | *       * | | | *  | | | *       * | | |*
>Victoria, B.C.,         `*#*'           `*#*'    `*#*'           `*#*'
>Canada. V8W 3N5.
>Tel (250) 472-4067, Fax(250) 472-4075
>http://darwin.ceh.uvic.ca/students/gstuart/
>
>

Didn't you treat the lysate with the nucleases?

Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu



More information about the Methods mailing list