Deep Vent Pol (ssDNA)

Nick Jacobsen jacobsen at cf.ac.uk
Fri Feb 7 07:25:39 EST 1997


Hi,
   I have used Deep Vent in regular PCR (although NEB do not advocate 
it). I have always found that the 2mM MgSO4 is not right for the PCRs I 
have done. I always use Taq buffer and 1.5mM MgCl2. This works fine. Ive 
never found any use for the buffers and MgSO4 solutions supplied with 
Deep Vent.

cheers

Nick.




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