High G/C PCR

Dr. Duncan Clark duncan at genesys.demon.co.uk
Fri Feb 7 08:23:30 EST 1997


Try using betaine at say 1M final in your PCR buffer. Make up a 5M stock
of Sigma B2754. Set up PCR's with 0.5, 1.0, 1.5M etc. It should help.
DMSO to 5% final may also help. I prefer to use new DMSO ie not some
that has been sitting around for a couple of years since first opened.

Duncan 
--------------------------------------------------------------------------------
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288
http://www.dnamp.com
http://www.genesys.demon.co.uk



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