Q: purifying lambda phage from extraneous DNA
pmitsis at seq.sarnoff.com
Fri Feb 7 15:57:31 EST 1997
In Article <32fbc017.83078841 at news.uvic.ca>, gstuart at uvic.ca (Greg Stuart)
>I am looking for suggestions on how to purify (or separate) lambda
>phage particles from DNA left-over from an in vitro packaging
>reaction. Any ideas? Thanks, Greg :)
>Greg Stuart gstuart at uvic.ca
>Centre for Environmental Health . .*#*. .*#*. .*#*.
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The classic way is using a CsCl gradient since the densities are very
different. Check out Maniatis for details. You probably can use a preformed
step gradient to shorten the run times.
Paul G. Mitsis
*** Leading Genomics into the New Millenium****
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