In Article <32fbc017.83078841 at news.uvic.ca>, gstuart at uvic.ca (Greg Stuart)
>I am looking for suggestions on how to purify (or separate) lambda
>phage particles from DNA left-over from an in vitro packaging
>reaction. Any ideas? Thanks, Greg :)
>Greg Stuart gstuart at uvic.ca>Centre for Environmental Health . .*#*. .*#*. .*#*.
>Department of Biology * | | | * | | | * * | | | * | | | *
>University of Victoria * | | | * * | | | * * | | | * * | | | *
>P.O. Box 3020 * | | | * * | | | * | | | * * | | |*
>Victoria, B.C., `*#*' `*#*' `*#*' `*#*'
>Canada. V8W 3N5.
>Tel (250) 472-4067, Fax(250) 472-4075
The classic way is using a CsCl gradient since the densities are very
different. Check out Maniatis for details. You probably can use a preformed
step gradient to shorten the run times.
Paul G. Mitsis
*** Leading Genomics into the New Millenium****
e-mail - pmitsis at seq.sarnoff.com
Voice - 609-452-6033 ext. 20
FAX - 609-452-5955