Q: purifying lambda phage from extraneous DNA

Paul Mitsis pmitsis at seq.sarnoff.com
Fri Feb 7 15:57:31 EST 1997


In Article <32fbc017.83078841 at news.uvic.ca>, gstuart at uvic.ca (Greg Stuart)
wrote:
>I am looking for suggestions on how to purify (or separate) lambda
>phage particles from DNA left-over from an in vitro packaging
>reaction. Any ideas? Thanks, Greg  :)
>Greg Stuart    gstuart at uvic.ca
>Centre for Environmental Health .   .*#*.            .*#*.   .*#*.
>Department of Biology    * | | | * | | | *        * | | | * | | | *
>University of Victoria * | | | *   * | | | *    * | | | *   * | | | *
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>Victoria, B.C.,         `*#*'           `*#*'    `*#*'           `*#*'
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>Tel (250) 472-4067, Fax(250) 472-4075
>http://darwin.ceh.uvic.ca/students/gstuart/

    The classic way is using a CsCl gradient since the densities are very
different. Check out Maniatis for details. You probably can use a preformed
step gradient to shorten the run times.

Yours
Paul Mitsis 
Paul G. Mitsis
Senior Scientist
SEQ Ltd.  
*** Leading Genomics into the New Millenium****

e-mail -  pmitsis at seq.sarnoff.com
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