Qustion on Ammonium sulfate and other precipitation methods
klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu
Sat Feb 8 13:58:00 EST 1997
In article <2cjcd5.en1.ln at wpxx02.toxi.uni-wuerzburg.de>,
krasel at wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) wrote:
->Rajesh Krishnamurthy (krishnam at umbc.edu) wrote:
->> I was reading about a problem with precipitating proteins using ammonium
->> sulfate. The protein that precipitated became insoluble. IS this expected
->> to happen with ammonium sulfate?
->It's not expected to happen but it may happen. Insoluble protein is
->most likely denatured.
Exactly. I'd just add that for most membrane-spanning proteins
denaturation upon precipitation should be expected.
/ /\ Dima Klenchin
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