In article <5d58jo$pp0 at newsstand.cit.cornell.edu>, "Christopher G.
Alpha" <cga3 at cornell.edu> writes
>>In article <528emb$3r0 at mserv1.dl.ac.uk>,
> "J.P. Hays" <jph5 at leicester.ac.uk> wrote:
>>In reply to Clemens Suter-Crazzolara :-
>>>>We too have a Perkin Elmer 9600 PCR machine that is 3 - 4 years old and
>we have problems with our machine (especially the cooling system). This
>to be a fault with the machine (we don't trust our 9600 for PCR anymore). As
>you mentioned the cost of servicing the machine is prohibitively expensive.
>All I can suggest is for people to try other (more reliable) PCR machines!
>>>>>>jph5 at le.ac.uk>>We've got the same machines too, running all day and night, and have
>experienced chiller failure as well...BUT its just a question (generally) of a
>little preventative maintainance every year or so. So have at thee! If you
>open up the machine, the cover tilts forward and locks into place, you will
>see the fluid pump and reservoir (clear brownish plastic on ours). Inside the
>reservoir, the cap unscrews (be careful to note the positions of the parts and
>the gaskets), there is a small cup-like filter for the coolant. The coolant
>BTW is basic auto antifreeze--20% E. glycol in dH2O, buy something good.
>Often the problem when you have chiller failure and the diagnostics indicate
>low flow as well--this filter is clogged. It just gets gummed up with
>deposits and bits of hose as they degrade over time. If it looks
>whiteish/opaque then take it to a sink and gently (its nylon and weak) scrub
>it with a test tube brush until clean and water flows thru it ok. Now flush
>the system out with dH2O by attaching a tube to the line coming into the
>reservoir cap and put the end of this to a beaker. (BE VERY CAREFUL here with
>fluids and the electronics put something around the work areas to soak up any
>spills from the reservoir etc...) Fill the open reservoir with dH2O and
>switch the machine on for a moment BUT DON'T LET THE FLUID LEVEL DROP BELOW
>THE BOTTOM OF THE RESERVOIR, otherwise you will lose the prime on the pump and
>get annoyed trying to prime it again (if you do: try backfilling the system),
>and then switch it off, refill, repeat until clear water runs out. Now flush
>the dH2O out with your 20% coolant solution the same way until that runs the
>right color. Put all the pieces back in and gaskets seated properly, and
>screw the cap on a LITTLE. Leave it loose and turn it on, the level will drop
>as air bubbles come out of the system. Turn it off, open it up, top up the
>reservoir, and repeat until you are sure its full and don't see any bubbles
>(some bubbles may get trapped-so gently tap on things and they'll pop free).
>Once satisfied tighten it all up and run the diagnostics again to verify that
>either you fixed it or that you have a pump or condenser problem instead...
>Plant Genetic Resources Unit
>Geneva, NY 14456
>>anyone need a handy tech out there?
>>Or hey since it costs at least 800.00 US to have them look at one of
>these, ship in to and from me and I'll do it for half, that's right!
>HALF!!! (just like some bad commercial, huh?) ;)