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Q: purifying lambda phage from extraneous DNA

Karl Fischer tyr-2 at bones.biochem.ualberta.ca
Sat Feb 8 18:24:46 EST 1997

> What about putting them a dialysis tube and concn. against solid PEG.
> The PEG will not go across the membrane but watch it closely - it is
> very easy to concn. to dryness!!

One caveat to this - if the presence of residual PEG can pose a problem in
downstream processing, use a PEG with an average molecular weight ten
times greater (at least) than the dialysis membrane exclusion limit. Found
out the hard way that PEG 6000/8000 WILL mobilize *into* a 5 kDa cut-off
dialysis bag...grumble, grumble.

Keep smilin'.

Karl the hepB guy

Karl Fischer
tyr-2 at bones.biochem.ualberta.ca

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