Q: How to remove small size RNA from total RNA in bacteria?

Chi-kuang Wen ckwen at expert.cc.purdue.edu
Sat Feb 8 10:00:47 EST 1997


Jinling Xie (jxie at ubvms.cc.buffalo.edu) wrote:
: Hi, there,

:     Anybosy happens to know if there is a way to remove small size 
: RNA from total RNA in bacteria except from gel, please reply to
: this account(small size means 5s rRNA, tRNA). Your information
: would be greatly appreciated.


: Jinling

What I knew was LiCl precipitation.  Small sizes of tRNA species precipitate
not as efficient as the RNAs with higher m.w..  HOwever, you might get rid of
some small mRNA , too.

Chi-Kuang
 



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