Silver Staining Reveals Contaminating Bands in Sample

David C. Logan david.logan at plant-sciences.oxford.ac.uk
Sat Feb 8 06:41:07 EST 1997


Huang Ke-xue (khuang at CHEMVX.CHEM.TAMU.EDU) wrote:

| I am looking for a way to get rid of 2 contaminating bands in one
| of the reagents in SDS Page sample buffer. These bands are visible on
| silver stained gels. I would appreciate hearing from anyone who has     	               
| had a similar experience and has solved this problem. Email replies 
can be sent to klnelson at uvic.ca

See "Removal of artifactual bands associated wwith the presence of 
2-mercaptoethanol in Two-dimensional PAGE" Beis, A and Lazou,A. 
Analytical Biochem. 190, 57-59 (1990). This worked a treat for me.
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david.logan at plants.ox.ac.uk

David C. Logan
Department of Plant Science
University of Oxford
South Parks Road
Oxford
OX1 3RB

Tel: (01865) 275024 (direct)
FAX: (01865) 275074
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