mAb Purification Difficulties

Tom Thatcher ttha at uhura.cc.rochester.edu
Thu Feb 6 20:54:47 EST 1997


In article <32F910CC.3FF3 at med.unc.edu>,
Patricia Keely <pkeely at MED.UNC.EDU> wrote:
>Dear Gods of the Internet please convey your great wisdom upon this
>troubled soul!
>
>I'm attempting to purify a mouse monoclonal IgG1 from tissue culture
>supernatent with little luck. I'm using proten A sepharose (HiTRAP column
>
>David D. Shock dsho at med.unc.edu

IgG1 binds very poorly to protein A.  I had pretty good luck with a
kit from Pierce, that used a Protein A derivative and some special
buffers.  I don't recall my % recovery but I got several mg from 
a few liters of culture media.

-- 
Tom Thatcher                          | You can give a PC to a Homo habilis,
University of Rochester Cancer Center | and he'll use it, but he'll use it
ttha at uhura.cc.rochester.edu           | to crack nuts.



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