Pfu polymerase in PCR

Dr. Duncan Clark duncan at genesys.demon.co.uk
Sun Feb 9 05:47:32 EST 1997


In article <5dgg5r$5fr at raven.cc.ukans.edu>, "Dr. Peter Gegenheimer"
<PGegen at kuhub.cc.ukans.edu> writes
>For that reason, NEB recommends an extension time of NO GREATER than 1 min per 
>kilobase, or 0.12 min for your 120-mer.

I believe that Pfu is less processive than Taq which is why everyone
recommendsto use longer extension times than 1min/kb with Pfu.

Duncan 
--------------------------------------------------------------------------------
The problem with being on the cutting edge is that you occasionally get 
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Dr. Duncan Clark
DNAmp Ltd.
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http://www.dnamp.com
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