Streptavidin and Biotin interactions

Diane Webster diane at PHYTON.OTAGO.AC.NZ
Sun Feb 9 18:25:35 EST 1997


Hi,

I am currently using biotin labelled oligo(dT) to capture my 
polyA tail potyvirus in streptavidin coated tubes for RT-PCR 
(Boehringer Mannheim's mRNA capture kit).

I would like to know more about how streptavidin and biotin interact.
  Specifically do they dissociate when heated during PCR (94 C)? 
And does the oligo(dT) dissociate from the biotin label during PCR?

General related details on streptavidin and biotin would be useful

Thanks in advance

Diane

Diane at phyton.otago.ac.nz
Botany Department
University of Otago
PO Box 56
Dunedin
New Zealand 



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