Aliquoting Antibodies for freezing - HELP!
bss3pk at surrey.ac.uk
Mon Feb 10 07:04:39 EST 1997
"Jen Robertson" <jvrobert at uoguelph.ca> wrote:
>I hope someone can give me advice on how to aliquot polyclonal IgG. I've
>been told to use 100% glycerin with added sodium azide. BUT the problem is
>that the glycerin is so gummy by the time the whole amount gets sucked into
>the pipette (250ul). I need help on this!!!! Any other suggestions. Please
>jvrobert at uoguelph.ca
Sorry about following-up on this post so late, but it has only just
made it onto my server.
... Not only that but I'm got going to answer your actual question.
I never freeze my antibodies. They are all stored at 2-4 deg C with
sodium azide added to 0.1%.
I am still using antibodies stored in this way which I made >15 years
ago (with little apparent loss in titre).
There are several reasons for adopting this strategy, and they
no freeze thawing - ever (and see below)
a (temporary) fridge breakdown is less catastophic than in freezer
access to as much or little of your stock as you need
moving labs with your precious stock is less fraught ..... :)
hope this helps
More information about the Methods