Chromosomal DNA carry-over in plasmid mini-preps
leach at bu.edu
Mon Feb 10 22:38:59 EST 1997
I'd check the neutralization solution (potassium acetate) used following the
maybe the ph or the concentration is off.....
usually the large 'gunk' is precipitated out during/following neutralization.
Michael Mangan (mmangan at iol.ie) wrote:
: Hello Netters,
: I have a very annoying problem with the carryover of chromosomal
: DNA in alkaline lysis minipreps, of midrange (60 kb) plasmids.
: Can anyone out there
: a) tell me what am I doing that is giving me this problem ?
: b) offer any solutions ?
I assume the lysis is going well.....does the solution still look viscous
following neutralization?? do you have much white ppt??
: Thanks in advance
..... Martin Leach Email:leach at bu.edu
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