sticky surfaces (fwd)

Karl Fischer tyr-2 at bones.biochem.ualberta.ca
Tue Feb 11 00:46:50 EST 1997


In article <5doovf$n1 at mserv1.dl.ac.uk>, Pradeep M Joshi
<pad29 at samba.tifrbng.res.in> wrote:

> > I am a new subscriber to the news group. I have run into a small problem. 
> > I want to do some surface staining of HaCat, an epithelial cell line for 
> > a FACS scan. The Problem now is that my cells dissociate only in th 
> > presence of trypsin and consequently most of my cell surface markers , 
> > receptors get chewed up. 

Have you ever tried using EDTA/EGTA in PBS to dissociate your cells?

Karl the hepB guy

-- 
Karl Fischer
tyr-2 at bones.biochem.ualberta.ca





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