painful gel loading???!!!!!!
Mark S. Rose
mark_rose at ncsu.edu
Mon Feb 10 07:39:01 EST 1997
In article <32FB5C55.43A at mindspring.com>, natldiagnos at mindspring.com wrote:
> Xudong Huang wrote:
> I have a painful problem about agarose gel loading. The DNA samples do
> not stay at the bottom of wells, intead, they float out of wells and
> diffuse into the buffer. The loading buffer I use is 6XBPB, the gel
> running buffer is 1X TBE. It is not because of air bubbles or oil in the
> samples. Could you let me know what is going on? Thank you.
> Xudong Huang
> email:xudong.huang at endo.mas.lu.se
While ethanol in your sample will cause loading problems I think your
problem is with your loading dye. A loading dye requires more than dye to
make it dense enough to sink to the bottom of a gel well. Try adding 40 %
sucrose or 30% glycerol to your loading dye.
Plant Pathology Dept.
More information about the Methods