painful gel loading???!!!!!!

Mark S. Rose mark_rose at
Mon Feb 10 07:39:01 EST 1997

In article <32FB5C55.43A at>, natldiagnos at wrote:

> Xudong Huang wrote:
> Hello,
> I have a painful problem about agarose gel loading. The DNA samples do
> not stay at the bottom of wells, intead, they float out of wells and
> diffuse into the buffer. The loading buffer I use is 6XBPB, the gel
> running buffer is 1X TBE. It is not because of air bubbles or oil in the
> samples. Could you let me know what is going on? Thank you.
> Xudong Huang
> email:xudong.huang at
While ethanol in your sample will cause loading problems I think your
problem is with your loading dye.  A loading dye requires more than dye to
make it dense enough to sink to the bottom of a gel well.  Try adding 40 %
sucrose or 30% glycerol to your loading dye.

Mark Rose
Plant Pathology Dept.
Raleigh,NC 27695-7616

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