Martin Cann (mjcann at MAIL.MED.CORNELL.EDU) wrote:
: I have a Northern which has a high background. The nylon has not dried
: out at any point and I have tried boiling 10mins in 0.5% SDS to remove
: the background but to no avail. Does anybody know how to strip this
: Northern or does it just have to sit in the freezer for a month?
: Martin Cann
:mjcann at med.cornell.edu
I found my formamide containing washing buffer to be more efficient for
stripping northerns than boiling the blot. Incubate the blot at 70 degrees C
for 2x 30min. (I do the stripping just like a hybridization in hybridization
tubes in a hybridization oven).
I guess any buffer with 50% formamide will work. If you want to try mine
here's the recipe:
120mM phosphate buffer pH6.8
If this doesn't work I would actually put the blot in the freezer.
Klaus Salger phone : +49 (0)89 5902 -502
Zoologisches Institut FAX : -450
AG Wetterauer e-mail: salger at zi.biologie.uni-muenchen.de