ssDNA (M13) extract. with silica?

Pascal Mertens Pascal.Mertens at fundp.ac.be
Mon Feb 10 10:52:31 EST 1997


Hello molbio netters,

I've prepared silica particles and accompanying lysis and wash buffers
according to Boom et al (J Clin Microbiol 28, 495-503, 1990) and have
purified with success genom DNA from blood samples for PCR.
(you can also see this protocol in pp19-20 of the PCR applications manual
from Boehringer Mannheim).

I wonder if it's possible to use this silica milk to purify M13 ssDNA
directly from culture supernatant (or from PEG-precipitated virions) for
sequencing in place of the usual phenol-chloroform extraction?
Does somebody have a nice protocol for that? (or any idea how to proceed).

Many thanks in advance
Pascal

-- 
Pascal Mertens
Laboratoire d'Immunologie et Microbiologie
URBM-FUNDP
61 Rue de Bruxelles
5000 Namur, BELGIUM
Phone: 32 81 724438
Fax: 32 81 724420



More information about the Methods mailing list