DNA recovery by phenol/chloroform extraction or GeneClean
Frank O. Fackelmayer
fof1 at chclu.chemie.uni-konstanz.de
Mon Feb 10 10:52:20 EST 1997
In article <Pine.GSO.3.95L.970209222612.22253B-100000 at uststf2>, Wilson
<netson at uxmail.ust.hk> wrote:
> Hello everybody,
> I would like to know which method can recovery DNA from agarose
> gel is better: Phenol/chloroform extraction or GeneClean? Which one can
> have higher yield of recovery and purity of DNA?
> Should I vortex the mixture during phenol/chloroform since I have
> heard that if the sticky ends DNA was vortexed, they will screw up
> together. What is the solution to this problem if DNA screw up? Can I
> solve it by incubation of the DNA at 65C for 15 min?
There are several kits or reagents available for purifying DNA fragments
from agarose gels. In my hands, GeneClean in not one of the best. However,
it´s much better then phenol extraction with molten agarose (Brrrrr!). I
use low melting point agarose and beta-agarase, which always performs well
and is not too expensive (yield >90% is normal).
Hope this helps,
PS: What do you mean with "DNA will screw up together"?
Dr. Frank O. Fackelmayer
Division of Biology
University of Konstanz
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