Have you tried a different brand of RNAse free DNAse? I had no problems
with Promega's RQ1 enzyme, and I am sure there have been posts here before
about problems with some brands. No affiliations, etc
Molecular Parasitology Unit Ph (02)95144043
University of Technology Fax (02)95144003
Sydney, AUSTRALIA michelle.gleeson at uts.edu.au
When you get to the end of your rope, tie a knot and hang on - F Roosevelt
On Mon, 3 Feb 1997, Francois Hatey wrote:
> Dear Netters,
>> Does somebody knows a good method to get undegraded RNA without DNA ?
> (we want to analyze these RNAs by RT-PCR and they are prepared from
> either tissues or cultured cells)
>> Classical method involves digesting by RNAse free-DNAse, but we get
> some RNA hydrolysis with this enzyme. Any advice ?
> What about alternative techniques such as lithium chloride precipitation
> associated with guanidium thiocyanate/phenol extraction (as described by
> Patanjali et al, PNAS 88, 1943-47, 1991) or something like that ?
>> Thanks in advance :-)
>> Francois Hatey
>> Laboratoire de Genetique Cellulaire, INRA
> Centre de Recherches de Toulouse, BP 27
> 31326 Castanet-Tolosan Cedex, France
>>hatey at toulouse.inra.fr>>