Inosine in degenerate primers

Dr. Douglas A. Johnson johnson at uottawa.ca
Tue Feb 11 17:32:49 EST 1997


In the archives there are many E-mails concerning the use of inosine as
a substitute for several bases when synthesizing degenerate primers. We
would like to use this approach too; however, there is little mention of
any problems or successes with the different, available
thermopolymerases. When it comes to the use of inosine-containing
degenerate primers, are all polymerases created equal??

Doug Johnson



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