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Enhancement of HRP substrate systems??

Per Mygind perm at biobase.dk
Tue Feb 11 13:18:41 EST 1997

Joep wrote:
> I would like to enhance my HRP based ELISA's, the use of a poly-HRP gives a
> good increase in signal, but I want more,
> Does anybody know any improvement by which I can obtain more signal?
> I already tried several substrates: ABTS, TMB, OPD; the best is TMB, but I
> need maximal sensitivity for detection of small peptides in serum/plasma.
> Thanks,
> Joep van Oers
> Amsterdam
> The Netherlands

You're right about the TMB, it works out to be the bewst cromogen
availible for HRP-detection. If this is not sensitive enough for you,
you might consider AP-konjugates as the are generally more sensitive.
I've tried an enhancement cromogen for detection of alkaline-phoshatase
conjugates that works really well (AMPAK-trademark, Danish company
called DAKO)


Per Mygind, Cand.scient
Institute of Medical Microbiology and Immunology
University of Aarhus, DENMARK

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