>> I would like to enhance my HRP based ELISA's, the use of a poly-HRP gives a
> good increase in signal, but I want more,
> Does anybody know any improvement by which I can obtain more signal?
>> I already tried several substrates: ABTS, TMB, OPD; the best is TMB, but I
> need maximal sensitivity for detection of small peptides in serum/plasma.
>> Joep van Oers
> The Netherlands
You're right about the TMB, it works out to be the bewst cromogen
availible for HRP-detection. If this is not sensitive enough for you,
you might consider AP-konjugates as the are generally more sensitive.
I've tried an enhancement cromogen for detection of alkaline-phoshatase
conjugates that works really well (AMPAK-trademark, Danish company
Per Mygind, Cand.scient
Institute of Medical Microbiology and Immunology
University of Aarhus, DENMARK