Error rate in Taq pol. 1:270?

Dr. Duncan Clark duncan at
Tue Feb 11 09:37:18 EST 1997

In article <3300740f.17308844 at>, Martin Offterdinger
<a8803349 at> writes
>I have read that using a lower nucleotide concentration e.g. 40uM
>instead of 200uM increases fidelity (but decreases efficiency and
>yield !!)

I think that was reported in NAR quite a while ago by Kunkel?
I'm meaning to get out all my Taq fidelity references today and read
them tonight if I get the chance!

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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