Hirt Extractions

Lawrence Mayer lmayer at unixg.ubc.ca
Wed Feb 12 01:24:37 EST 1997


I am trying to optimize my recovery of an ~12kb episomal plasmid with a
fairly low copy number from a human endothelial cell line.  I have tried a
few variations on the standard Hirt extraction technique but would like to
increase my recovery if possible.  If any one has any interesting tricks or
thoughts on this matter I would appreciate hearing from you.

	Thanks in advance,
				Anne



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