Inosine in degenerate primers

Fraser Dr N J njf16154 at
Wed Feb 12 06:25:49 EST 1997

johnson at ("Dr. Douglas A. Johnson") wrote:
>In the archives there are many E-mails concerning the use of inosine as
>a substitute for several bases when synthesizing degenerate primers. We
>would like to use this approach too; however, there is little mention of
>any problems or successes with the different, available
>thermopolymerases. When it comes to the use of inosine-containing
>degenerate primers, are all polymerases created equal??
>Doug Johnson

As mentioned in a recent posting, in my experience inosine containing primers
have worked well with Taq, but not at all with Pfu.

More information about the Methods mailing list