DNA-free RNA

Martin Offterdinger a8803349 at unet.univie.ac.at
Wed Feb 12 02:15:47 EST 1997


On Mon, 03 Feb 1997 18:33:42 -0800, Francois Hatey
<hatey at toulouse.inra.fr> wrote:

>Dear Netters,
>
>Does somebody knows a good method to get undegraded RNA without DNA ?
>(we want to analyze these RNAs by RT-PCR and they are prepared from 
>either tissues or cultured cells)
>
>Classical method involves digesting by RNAse free-DNAse, but we get 
>some RNA hydrolysis with this enzyme. Any advice ?
>What about alternative techniques such as lithium chloride precipitation 
>associated with guanidium thiocyanate/phenol extraction (as described by 
>Patanjali et al, PNAS 88, 1943-47, 1991) or something like that ?
>
>Thanks in advance :-)
>
>Francois Hatey
>
>Laboratoire de Genetique Cellulaire, INRA
>Centre de Recherches de Toulouse, BP 27
>31326 Castanet-Tolosan Cedex, France
>
>hatey at toulouse.inra.fr

Dear Francois
What about choosing your primers in a way that they span one or more
introns of genomic DNA-than you will get either no amplification of
DNA or the product will be much larger and should not be any problem.
Martin



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