Quantitative RT-PCR

J Kennie jkennie at GPU.SRV.UALBERTA.CA
Wed Feb 12 15:43:03 EST 1997


I am interested in using quantitative RT-PCR for measuring gene 
expression. I have been using RT-PCR to test for the presence/absence of
a few different mRNA in total RNA extracted from bovine lymphocytes and now I
would like to quantify the mRNA. I have read about a number of different
methods and variations of methods for quantitative RT-PCR. I was wondering
if anyone had any ideas/suggestions/opinions on the approach I should
take.

Jan Kennie
MSc. Student
University of Alberta
jkennie at gpu.srv.ualberta.ca




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