I will soon be embarking on a project to epitope-tag a putative membrane
protein in mouse small intestine, to be expressed in Xenopus oocytes and
mammalian cell lines. I am aware of three different kinds of epitopes
for which monclonals are available commercially: HA (from influenza
hemagglutinin), 9E10 (from human c-myc protein) and VSV-G (from
vesicular stomatitis virus glycoprotein).
Are there other epitopes available? What are the dis/ advantages?
It will be very helpful to me if I could hear from some personal
experience about any of the epitopes used.
I thank you all in advance.
Dept of Medicine
University of California, Irvine