Smearing of PCR product

XIAO-MIN SU sumini at welchlink.welch.jhu.edu
Wed Feb 12 12:20:36 EST 1997


Hi,

I would like some advice on a recent problem that I am facing. When I PCR 
out using template DNA, the products from a smear. My primers should 
amplify a 260bp band , but i am unable to see that band. A smear is 
present in the whole lane. I tried using all fresh reagents and this 
problem persists. My PCR conditions are 95 -1min, 55-30secs, 72-1min, 40 
cycles.I would very much appreciate any suggestions

ShriHari
kadkol at welchlink.welch.jhu.edu



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