At 02:30 PM 2/13/97 +0100, doering_t at mpimg-berlin-dahlem.mpg.de wrote:
>>I searching for protocols for staining proteins
>and/or nucleic acids with "Stains-All". I still have
>the substance, but I didn't find any protocol.
>We want to use it (if this is possible) for staining
>of proteins and RNA simultaneously in following the
>process of purification of a nucleic binding protein.
I do not have the reference handy, but this is what I used to do:
A: 25% 2-propanol
B: 0.1% StainsAll in formamide
C: 10mL B + 10mL formamide + 50mL 2-propanol + 3mL 1M Tris (pH8.8) ---> to 200mL with dH2O.
Following electrophoresis: Agitate gel in 'A' for 15 min. Stain for a few hrs to O/N in C. Destain in dH2O.
The sensitivity is said to be about 300pmole of -PO4(+3).
Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu