Mechanism of EMS Action?

martin LEACH leach at bu.edu
Thu Feb 13 14:54:16 EST 1997


Namjin Chung (nc1 at acpub.duke.edu) wrote:
: Hi, all!

: EMS (MMS) is an alkylating agent, and widely used in mutagenesis.  I
: wonder how this mutagen work on DNA: probably by modifying bases so that
: mismatches would happen? Is there any other molecules, proteins or lipids,
: being alkylated by EMS?  If there is, how does EMS affect their biological
: activities/functions?

MMS (methyl-methane sulfonate)

is mutagenic by the following way:

causes alkaylation of guanosine at the 7 position with resulting tautomeric
shift.


nasty stuff!

M


: Any comments would be greatly appreciated.  TIA.


: Namjin Chung, who knows little and asks a lot!
: Program in Molecular Cancer Biology
: Duke University Medical Center
: Durham, North Carolina

--
.....          Martin Leach                Email:leach at bu.edu
   _|____      Dept. of Pharmacology       Phone: (617) 638-5323
   / o  /      Boston Univ. School of Med. Fax:   (617) 638-4329
 _/  |-/__==/  80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118            "Not the old underpants
               USA                                on your
                                             head.....WIBBLE"

My homepage: http://www.tradesmart.com/martin/Leach.html
Webmaster of the Biotech Rumor mill: http://www.tradesmart.com/rumor



More information about the Methods mailing list