Q: How to remove small size RNA from total RNA in Bacteria?

martin LEACH leach at bu.edu
Thu Feb 13 13:48:35 EST 1997


I remember seeing in the Qiagen handbook the elution profile of different
moleculr species....with varying NaCl concs.

never tried it...but it looks like 0.7M NaCl for tRNA and 0.95M NaCl for rRNA
at Ph7.0 works with the qiagen resin...

give 'em a call

M

p.s. not affiliated with Qiagen....but am happy using their products.

Jinling Xie (jxie at ubvms.cc.buffalo.edu) wrote:
: Hi, there,


:       Anybody happens to know how to remove small size RNA from
: total RNA in bacteria (such as 5s RNA, tRNA), please reply
: to this account. Thanks for your help!

: Jinling

--
.....          Martin Leach                Email:leach at bu.edu
   _|____      Dept. of Pharmacology       Phone: (617) 638-5323
   / o  /      Boston Univ. School of Med. Fax:   (617) 638-4329
 _/  |-/__==/  80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118            "Not the old underpants
               USA                                on your
                                             head.....WIBBLE"

My homepage: http://www.tradesmart.com/martin/Leach.html
Webmaster of the Biotech Rumor mill: http://www.tradesmart.com/rumor



More information about the Methods mailing list