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Quick transformation protocol wanted

Charles Brockhouse chbrock at iastate.edu
Thu Feb 13 10:35:15 EST 1997


A.P.N. de Boer wrote:
> 
> Hi,
> Once upon a time, I learned a very quick (and dirty) transformation
> protocol. It could be used to transform strains with plasmid DNA (which
> doesn't need a high transformation efficiency). It was very quick and and
> just entailed mixing some bacteria (colony from plate or pellet or
> anything), DNA and the transformation mix and the mix could be plated.
> I can't find the protocol anymore (moved labs). Does anyone here know of
> such a protocol ?
> Thanks, Thon de Boer

Try DNA cloning: a practical approach, vol I.  IRL Press.
-- 
Charles L. Brockhouse
Insect Genetics, Entomology
Iowa State University
Ames, IA., 50011-3222

Tel: (515) 294-4570
Fax: (515) 294-5957



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