A.P.N. de Boer wrote:
> Once upon a time, I learned a very quick (and dirty) transformation
> protocol. It could be used to transform strains with plasmid DNA (which
> doesn't need a high transformation efficiency). It was very quick and and
> just entailed mixing some bacteria (colony from plate or pellet or
> anything), DNA and the transformation mix and the mix could be plated.
> I can't find the protocol anymore (moved labs). Does anyone here know of
> such a protocol ?
> Thanks, Thon de Boer
Try DNA cloning: a practical approach, vol I. IRL Press.
Charles L. Brockhouse
Insect Genetics, Entomology
Iowa State University
Ames, IA., 50011-3222
Tel: (515) 294-4570
Fax: (515) 294-5957