Chromosomal DNA carry-over in plasmid mini-preps

DSF 76042.532 at CompuServe.COM
Thu Feb 13 12:38:30 EST 1997


You might be being a little too rough during lysis, or maybe 
lysing too long. Try gentle inversion, just enough to mix. Also, 
when you remove your aqueous layer after extraction, leave behind 
a little something for the fairies. If you must be greedy, just 
extract the organic fraction again. I hope this helps.
Lynette



More information about the Methods mailing list