Smearing of PCR product

Landry Pierre-Alexandre landryp at ERE.UMontreal.CA
Thu Feb 13 11:45:38 EST 1997


XIAO-MIN SU <sumini at welchlink.welch.jhu.edu> writes:

>Hi,

>I would like some advice on a recent problem that I am facing. When I PCR 
>out using template DNA, the products from a smear. My primers should 
>amplify a 260bp band , but i am unable to see that band. A smear is 
>present in the whole lane. I tried using all fresh reagents and this 
>problem persists. My PCR conditions are 95 -1min, 55-30secs, 72-1min, 40 
>cycles.I would very much appreciate any suggestions



Try with 30-35 cycles.

Ax



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