DNA recovery by phenol/chloroform extraction or GeneClean

Steven Goldberg goldberg at bms.com
Thu Feb 13 11:30:24 EST 1997

In article <Pine.GSO.3.95L.970209222612.22253B-100000 at uststf2>, Wilson
<netson at uxmail.ust.hk> wrote:

> Hello everybody,
>         I would like to know which method can recovery DNA from agarose
> gel is better: Phenol/chloroform extraction or GeneClean?  Which one can
> have higher yield of recovery and purity of DNA?
>         Should I vortex the mixture during phenol/chloroform since I have
> heard that if the sticky ends DNA was vortexed, they will screw up
> together.  What is the solution to this problem if DNA screw up?  Can I
> solve it by incubation of the DNA at 65C for 15 min?
> Wilson

After lots of problems using GeneCleaned DNA in ligations, I switched to
the QIAquick gel extraction kit and have had very good luck in terms of
yield and purity.


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