In article <Pine.GSO.3.95L.970209222612.22253B-100000 at uststf2>, Wilson
<netson at uxmail.ust.hk> wrote:
> Hello everybody,
> I would like to know which method can recovery DNA from agarose
> gel is better: Phenol/chloroform extraction or GeneClean? Which one can
> have higher yield of recovery and purity of DNA?
> Should I vortex the mixture during phenol/chloroform since I have
> heard that if the sticky ends DNA was vortexed, they will screw up
> together. What is the solution to this problem if DNA screw up? Can I
> solve it by incubation of the DNA at 65C for 15 min?
After lots of problems using GeneCleaned DNA in ligations, I switched to
the QIAquick gel extraction kit and have had very good luck in terms of
yield and purity.