XGAL & IPTG -- how low can you go?

[martin LEACH]

Ann L. Williams (awilliams at biochem.okstate.edu) wrote:
: We have good luck with applying the IPTG & X-Gal to the surface only 
: of the plates.  As always TIME=$.  I mix small amounts of my two 
: stocks and spread 100ul of this stock on the plates circa half an hour 
: before I plate my cells (spread or streak).  The stocks we use:
:    2%   X-Gal (in pmsf)

Why do you choose PMSF.....this is nasty nasty stuff (protease inhibitor?)

I use DMF as the solvent...

M

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