stability of GFP in E.coli?

Wolfgang Schechinger wgschech at med.uni-tuebingen.de
Thu Feb 13 11:07:48 EST 1997


Hi netters,
I wonder how long GFP (S65T, 
eucaryotic codons) expressed in E. coli might be stable i.e. 
detectable by fluorescence, when the bacteria are stored in the 
fridge?

Has anyone yet tried to detect GFP in the protein-waste of minipreps 
(the pellet after the neutralization step (NOT the DNA itself)?

Any comments are welcome!


Wolfgang


---------!all possible disclaimers apply!-----------
                                     
Wolfgang Schechinger
University of Tuebingen
email: wgschech at med.uni-tuebingen.de

http://www.medizin.uni-tuebingen.de/~wgschech/research.htm

public PGP key is avilable on request	



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