XGAL & IPTG -- how low can you go?

Ann L. Williams awilliams at biochem.okstate.edu
Thu Feb 13 10:16:58 EST 1997


We have good luck with applying the IPTG & X-Gal to the surface only 
of the plates.  As always TIME=$.  I mix small amounts of my two 
stocks and spread 100ul of this stock on the plates circa half an hour 
before I plate my cells (spread or streak).  The stocks we use:
   2%   X-Gal (in pmsf)
  100mM IPTG

The final is equivalent to 83 ul 2% X-Gal, 17 ul 100 mM IPTG/plate.
I have not tried to see how much less I could use, but believe that it 
could be lowered some.
-- 
Ann L. Williams
Oklahoma State University
Biochemistry & Molecular Biology
246 Noble Research Center
Stillwater OK 74078-3035

Phone:  (405) 744-9340
Fax:    (405) 744-7799
e-mail: awilliams at biochem.okstate.edu



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