Low infection of WEHI231 cell line with eco/ampho retrovirus

tim timt at itsa.ucsf.edu
Thu Feb 13 20:47:17 EST 1997


I have been trying to infect WEHI231 (an immature B cell line) with
ecotropic Moloney Murine Leukemia Virus-based retrovirus carrying cDNA
sequences.  What I got was some hundreds infectants (thru selection via
puro resistance on the vector or thru lacZ as reporter cDNA) out of
millions of cells.  However, the same virus prep was titered over 10-5 in
NIH3T3 cells.

I also tried amphotropic vector and the infection efficiency was not
dramatically boosted.

Any suggestions, tips, will be appreciated.

Sincerely puzzled and confused,

Tim



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