I have been trying to infect WEHI231 (an immature B cell line) with
ecotropic Moloney Murine Leukemia Virus-based retrovirus carrying cDNA
sequences. What I got was some hundreds infectants (thru selection via
puro resistance on the vector or thru lacZ as reporter cDNA) out of
millions of cells. However, the same virus prep was titered over 10-5 in
I also tried amphotropic vector and the infection efficiency was not
Any suggestions, tips, will be appreciated.
Sincerely puzzled and confused,