Transfection

[Steve Cohen]

Fellow netters,

I'm want to find which method of transfection results in the highest
percentage of cells taking up DNA. I want to do a transient transfection,
followed by a western. At present my western signal is very weak if present
at all. I think this is due to only a small number of cells being
transfected. I am presently using chicken embryo fibroblasts.

Your thoughts on this would be helpful.

Steve