"Oligonucleotide purification method??"

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Thu Feb 13 15:55:24 EST 1997


Balwi (M.Albalwi at sheffield.ac.uk) wrote:
> I have labelled some oligonucleotide with dig-11-dutp at 3'end, but I did
> not know which best method to clean unincorporated nucleotide
> (dig-11-dutp). Is it by using preceiptation method(Na-acetat/ethanol) 
> or by using spun colum. If you think you have idea please e-mail me.

I can't speak for dig-labeled nucleotide, but I recall a paper (?) where
it was demonstrated that spun columns were much more effective than
precipitation or ultrafiltration to clean up 32P-labeled oligos. I
purified oligos via PAGE, with very good results.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP3 */
/* "Science is the game we play with God to find out what His rules are."  */



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