In article <32FF6942.4377 at midplains.net>, Les Hoffman
<lhoffman at midplains.net> wrote:
>In 1990 Biotechniques vol.8 (6), pp. 674-678, R.A. McGraw et al.
>presented a method for calculating the melting point of oligos based on
>nearest-neighbor interactions of the bases. We have found the
>hybridization temperatures calculated manually with their methods give
>very accurate estimates of the Tm of oligos around 20 bases long. We
>cannot, however, find a source for the software for the algorithms for
>mean stacking temperature and "T50-hyb", the Tm. Doing these
>calculations by hand seems a little ridiculous. Does anyone out there
>know where we can download or obtain the software?
There are commercial packages that do it, as well as the free Whitehead
Primer program, but I find all these to be cumbersome. I prefer the
Minnesota web site that performs the calculation:
By the way, the original reference for the method is: Breslauer et al.,
Proc. Nat. Acad. Sci. 83, 3746-50, 1986.
Good luck with your new products, and say Hi to Gary Dahl for me.
Michael Finney, PhD | | mikef at mjr.com
MJ Research, Inc. | /\/\\_\ /\_\ | 617 370 8112
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