how to identify a gene on human chromsome 11
Soonmyoung Paik, M.D.
spaik at asri.edu
Fri Feb 14 20:33:05 EST 1997
You may be interested in reading the article by Jeff Trent's group
published in Nature Genetics December 1996 issue. They used cDNA
microarray to identify the genes whose expression have changed when
chromosome 6 was inserted into tumorigenic cells which resulted in
suppression of tumorigenic phenotype.
Title: Use of a cDNA microarray to analyse gene expression patterns in
Authors: DeRisi J, et al
Source: Nature Genetics 14:457-460
K-John Cheung wrote:
> hi, our lab has previously found that insertion of human chromosome 11 into bladder cancer cells can protect them from H2O2 damage as assessed
> staining technique. although the levels of catalse (the catalase gene is situated on chromosome 11) have clearly increased in hte cels with
> insert, we have reasons to believe that other regions of this chromsome are
> responsible for this protective phenotype. now, we have used differential
> display to blindly find something that might be of interest to us but so far, nono luck. even if we find something, that may not be on
chromosome 11. so if we
> insist on finding that gene(s) on this chromosome, what techniques are
> RDA, representation difference analysis, sounds kinda good but i am sure how
> reliable and productive it is.
> any comments would be appreciated.
> BC Cancer Research Center & Simon Fraser University, Carcinogenesis Laboratory
> BC, Canada
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