j303nih at dsmc.or.kr
Fri Feb 14 03:39:54 EST 1997
Thomas Adam wrote:
> Does anybody out there have a simple but efficient protocol to rid plasmid DNA of
> LPS contamination? Thank you . Thomas
Quiagen plasmid prep is reported to reduce LPS contamination
see Ref. Human Gene Therapy 6:317-323(1995)
Bacterial LPS copurified with plasmid DNA : Implications for
Animal Models and Human Gene Therapy
Wicks et al.
DNA Etox reagent from Sterogene Bioseparations, Inc.(sterogene at aol.com)
removes endotoxin from DNA preparations.
Hope it helps.
Dep. of Immunology
Keimyung Univ. Medical School
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