DNA recovery by phenol/chloroform extraction or GeneClean

Betsy L. Alberty alsbyte1 at well.com
Fri Feb 14 22:47:49 EST 1997


I have a lot of customers oSteven Goldberg wrote:
> 
> In article <Pine.GSO.3.95L.970209222612.22253B-100000 at uststf2>, Wilson
> <netson at uxmail.ust.hk> wrote:
> 
> > Hello everybody,
> >         I would like to know which method can recovery DNA from agarose
> > gel is better: Phenol/chloroform extraction or GeneClean?  Which one can
> > have higher yield of recovery and purity of DNA?
> >         Should I vortex the mixture during phenol/chloroform since I have
> > heard that if the sticky ends DNA was vortexed, they will screw up
> > together.  What is the solution to this problem if DNA screw up?  Can I
> > solve it by incubation of the DNA at 65C for 15 min?
> >
> > Wilson
> 
> After lots of problems using GeneCleaned DNA in ligations, I switched to
> the QIAquick gel extraction kit and have had very good luck in terms of
> yield and purity.
> 
> Steve
> >I have a lot of customers out here who absolutely love Epicentre's 
GELase.
Betsy Alberty / ALSBYTE Biotech



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