DNA recovery by phenol/chloroform extraction or GeneClean
Betsy L. Alberty
alsbyte1 at well.com
Fri Feb 14 22:47:49 EST 1997
I have a lot of customers oSteven Goldberg wrote:
> In article <Pine.GSO.3.95L.970209222612.22253B-100000 at uststf2>, Wilson
> <netson at uxmail.ust.hk> wrote:
> > Hello everybody,
> > I would like to know which method can recovery DNA from agarose
> > gel is better: Phenol/chloroform extraction or GeneClean? Which one can
> > have higher yield of recovery and purity of DNA?
> > Should I vortex the mixture during phenol/chloroform since I have
> > heard that if the sticky ends DNA was vortexed, they will screw up
> > together. What is the solution to this problem if DNA screw up? Can I
> > solve it by incubation of the DNA at 65C for 15 min?
> > Wilson
> After lots of problems using GeneCleaned DNA in ligations, I switched to
> the QIAquick gel extraction kit and have had very good luck in terms of
> yield and purity.
> >I have a lot of customers out here who absolutely love Epicentre's
Betsy Alberty / ALSBYTE Biotech
More information about the Methods